mouse conditioning chamber  (Med Associates Inc)

Journal: Genes

Article Title: Effects of Paraquat, Dextran Sulfate Sodium, and Irradiation on Behavioral and Cognitive Performance and the Gut Microbiome in A53T and A53T-L444P Mice

doi: 10.3390/genes15030282

Figure Lengend Snippet: ( A ) In the open field, activity levels were higher in A53T than A54T-L444P mice ( F (1,81) = 4.091, * p = 0.046). ( B ) In the rotorod test, there was a genotype × treatment interaction ( F (1,81) = 7.773, p = 0.007). DSS impaired rotorod performance in A53T-L444P mice ( F (1,45) = 9.228, *** p = 0.004), but not in A53T mice. ( C ) DSS reduced spontaneous alternation in A53T-L444P mice ( F (1,45) = 7.191, * p = 0.01). ( D ) A53T-L44P mice showed reduced activity levels in the Y-maze ( F (1,81) = 5.336, * p = 0.023). ( E ) During the baseline period in the fear conditioning test, activity levels were lower in A53T-L44P than A53T mice ( F (1,81) = 7.565, ** p = 0.007). ( F ) In males, A53T-L44P mice showed lower activity levels than A53T mice during the baseline period ( F (1,41) = 6.727, * p = 0.013). ( G ) In females, there was a treatment × genotype interaction ( F (1,40) = 4.168, p = 0.048). DSS increased activity level in A53T-L44P females ( F (1,22) = 5.119, * p = 0.034). ( H ) For the freezing levels during the ISI, there was a genotype × treatment × sex interaction ( F (1,81) = 4.661, p = 0.034). ( I ) A53T-L44P mice had lower activity levels than A53T mice during the ISI ( F (1,81) = 4.564, * p = 0.036). ( J ) When activity levels during the tones were analyzed, there was a genotype × treatment × sex interaction ( F (1,81) = 8.199, p = 0.005). ( K ) A53T-L44P mice had lower activity levels than A53T mice during the contextual fear memory test ( F (1,85) = 4.677, * p = 0.033). Circles: A53T; squares: A53T-L444P. Open symbols: H 2 O; filled symbols: DSS.

Article Snippet: Contextual and cued fear conditioning were assessed (Med Associates mouse fear conditioning system (PMED-VFC-NIR-M, Med Associates, St. Albans, Vermont and Med Associates VideoFreeze automated scoring system).

Techniques: Activity Assay

Journal: Genes

Article Title: Effects of Paraquat, Dextran Sulfate Sodium, and Irradiation on Behavioral and Cognitive Performance and the Gut Microbiome in A53T and A53T-L444P Mice

doi: 10.3390/genes15030282

Figure Lengend Snippet: Summary of behavioral data of studies 1, 3, and 4 1 .

Article Snippet: Contextual and cued fear conditioning were assessed (Med Associates mouse fear conditioning system (PMED-VFC-NIR-M, Med Associates, St. Albans, Vermont and Med Associates VideoFreeze automated scoring system).

Techniques: Activity Assay, Saline

Journal: Frontiers in Neuroscience

Article Title: Chronic interleukin-6 mediated neuroinflammation decreases anxiety, and impaires spatial memory in aged female mice

doi: 10.3389/fnins.2023.1267818

Figure Lengend Snippet: GFAP-IL6 mice display impaired intermediate-term memory but not associative learning and memory. (A) Percentage (%) of time spent in the arms of the YM by GFAP-IL6 and WT mice. Time (s) ‘ freezing’ for FC during (B) Conditioning Day, (C) Context Day, and (D) Cue Day for GFAP-IL6 and WT mice. Data analyzed with one-way ANOVA and one-sample t -test (theoretical value = 33) for the YM and two-way RM ANOVA for the FC, and presented as means ± S.E.M. Main effect of ‘genotype’ indicated by asterisks (** p < 0.01), and significant difference from theoretical value in the t -test indicated by ‘at’ symbols ( @ p ≤ 0.05) ( n = 15 WT;12 GFAP-IL6).

Article Snippet: The apparatus was a mouse fear conditioning chamber (VFC-008, Med Associates, 29.5 cm × 24.5 cm × 21 cm), and methods have been published previously in our laboratory ( ; ).

Techniques: